Membrane fluidity and lipid content of human spermatozoa selected by swim-up method

Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa.

Semen cryopreservation is an important procedure in the treatment of human infertility. However, the ability of spermatozoa to survive freeze/thaw processes varies between patients. Cryopreservation-induced stress may result in membrane injury with consequent loss of sperm motility and viability. We investigated the relationship between the physico-chemical state of the human sperm membranes an...

Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method

OBJECTIVE In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo were modeled in vitro. Available healthy sperm obtained in the swim-up procedure using HA were investigated. MATERIALS AND METHODS This observation...

Lipid fluidity and membrane protein dynamics.

Membrane fluidity plays an important role in cellular functions. Membrane proteins are mobile in the lipid fluid environment; lateral diffusion of membrane proteins is slower than expected by theory, due to both the effect of protein crowding in the membrane and to constraints from the aqueous matrix. A major aspect of diffusion is in macromolecular associations: reduction of dimensionality for...

Lipid molecular species retailoring and membrane fluidity.

on cell morphology, i.e. the formation of stomatocytes, when the native mixed-acid PC species in the monolayer are replaced by (1,2-dilinoleoyl)-PC (Fig. 1). This species has a pronounced cone shape because of a larger cross-section at the end of its hydrophobic tails. Consequently, the outer monolayer, and thereby the whole membrane, will bend inwards. Further support for the above hypothesis ...

Control of membrane lipid fluidity by molecular thermosensors.